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BRAIN Publication-derived

Frontotemporal Dementia Cortical Organoids

Full SOP with Academic or a plan

Source Cordella et al., 2025 · Sapienza University of Rome · 10.1002/alz.70419

Authors: Federica Cordella, Lorenza Mautone, Debora Salerno, Lucrezia Tondo, Silvia Ghirga, Chiara D'Antoni, Erika Parente, Maria Anele Romeo, Mara Cirone, Paola Bezzi, Silvia Di Angelantonio Duration: 100 days 4 phases Cell source: Human iPSC

Abstract

This protocol generates patterned 3D cortical organoids from isogenic control and MAPT IVS10+16 mutant human iPSC lines to model frontotemporal dementia. The protocol combines neural induction, neural differentiation, and terminal maturation over 100 days, with optional bezafibrate treatment to assess mitochondrial function and tau-related pathology rescue.

Cell source
Human iPSC
Application
Disease modeling

Protocol overview

12 steps across 4 phases

Phase 1 · iPSC Maintenance and Cortical Organoid Initiation D0-D8
  1. a iPSC Culture Maintenance
  2. b iPSC Dissociation and Seeding into AggreWell800
  3. c Neural Induction Medium (NIM) Initiation
  4. d Daily Medium Changes During Neural Induction (D1-D8)
  5. e Organoid Harvest at D8
Phase 2 · Neural Differentiation and Maturation D8-D30
  1. a Neural Differentiation Medium (NDM) Supplementation (D8-D30)
  2. b Transition to Neuronal Maturation Factors at D30
  3. c Medium Composition Change at D50
Phase 3 · Late Maturation and Bezafibrate Treatment (Optional) D50-D100
  1. a Maintain Culture through D100
  2. b Bezafibrate (BZ) Treatment (D70-D100, Optional)
Phase 4 · Organoid Dissociation to 2D Cortical Cultures (Optional) D70
  1. a Harvest and Dissociate Organoids
  2. b Plate Neurons onto Coated Dishes

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Attribution

This SOP was written by Organthis based on the published method in Cordella et al., 2025. Once the originating laboratory has confirmed it, the SOP is marked Verified.

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