Skip to content
← Back to browse
BRAIN Publication-derived

Brain-Region-Specific Organoids Using Mini-Bioreactors (SpinΩ)

Source Qian et al. · Johns Hopkins University School of Medicine, Baltimore, MD, USA · 10.1016/j.cell.2016.04.032

👤 Qian X, Nguyen HN, Song MM, Hadiono C, Ogden SC, Hammack C, Yao B, Hamersky GR, Jacob F, Zhong C, Yoon KJ, Jeang W, Lin L, Li Y, Thakor J, Berg DA, Zhang C, Kang E, Chickber M, Nauen D, Ho CY, Wen Z, Christian KM, Shi PY, Maher BJ, Wu H, Jin P, Tang H, Song H, Ming GL ⏱ 100 days 📋 4 phases 🧫 Human iPSC

Abstract

Qian et al. developed the SpinΩ miniaturized spinning bioreactor system enabling cost-effective, scalable generation of brain-region-specific organoids including forebrain, midbrain, and hypothalamus tissues from human iPSCs. The platform was applied to model Zika virus infection, demonstrating preferential viral tropism for neural progenitor cells.

Cell source
Human iPSC
Application
Region-Specific Brain Modeling

Protocol overview

12 steps across 4 phases

iPSC Preparation and Embryoid Body Formation Days 1-7
  1. 1 iPSC Colony Dissociation
  2. 2 Transfer Colonies to Ultra-Low Attachment Plate
  3. 3 Medium Replacement with Induction Medium
Matrigel Embedding and Pre-patterning Days 7-14
  1. 4 Embed Organoids in Matrigel
  2. 5 Mechanically Dissociate from Matrigel
Spinning Culture and Differentiation Days 14-100
  1. 6 Transfer to SpinU Bioreactor
  2. 7 Medium Changes During Growth
  3. 8 Switch to Maturation Medium at Day 71
  4. 9 Continue Culture in Maturation Medium
ZIKV Infection (Optional Disease Model) Day 14, 28, or 80 (24 hours exposure)
  1. 10 Prepare ZIKV Inoculum
  2. 11 Apply ZIKV to Organoids
  3. 12 Replace Medium and Continue Culture

Full SOP

🔬

Create a free account to access this protocol

Join OrganMatch to unlock step-by-step procedures, reagent concentrations, QC checklists, and downloadable batch record templates.

Create free account

Already registered? Log in

Attribution

This SOP was authored by Organthis based on the published method in Qian et al.. The originating laboratory holds no rights in this SOP and has not endorsed it unless marked Verified.

This wording is awaiting legal review.

Something wrong with this entry? Report an issue with this protocol

Need a commercial licence?
Use this protocol in your therapeutic or diagnostic pipeline.
Request Commercial Licence