BRAIN Publication-derived

Individual Brain Organoids Reproducibly Form Cell Diversity

Source Velasco et al. · Harvard University, Cambridge, MA, USA · 10.1038/s41586-019-1289-x

👤 Velasco S, Kedaigle AJ, Simmons SK, Nash A, Rober M, Cayber B, Park J, Sber AJ, Villalobos E, Rogber CJ, Gilhool K, Esteves A, Chandrasekaran S, Pfister R, Lehtinen MK, Regev A, Arlotta P ⏱ 86400 min 🧫 Human iPSC / Human ESC

Abstract

Velasco et al. addressed the critical reproducibility challenge in brain organoid research by developing an optimized guided differentiation protocol that generates cortical organoids with consistent cell type diversity across individual organoids, batches, and iPSC lines. Single-cell RNA-sequencing over a 6-month culture period confirmed the reliable generation of all major cortical neuron subtypes.

Cell source

Human iPSC / Human ESC

Application

Reproducible Cortical Organoid Production

Full SOP

🔬

Create a free account to access this protocol

Join OrganMatch to unlock step-by-step procedures, reagent concentrations, QC checklists, and downloadable batch record templates.

Create free account

Already registered? Log in

Attribution

This SOP was authored by Organthis based on the published method in Velasco et al.. The originating laboratory holds no rights in this SOP and has not endorsed it unless marked Verified.

This wording is awaiting legal review.

Something wrong with this entry? Report an issue with this protocol

Need a commercial licence?

Use this protocol in your therapeutic or diagnostic pipeline.

Request Commercial Licence