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BRAIN Publication-derived
Cortical Organoids
No SOP yet
Source Velasco et al. · Harvard University, Cambridge, MA, USA · 10.1038/s41586-019-1289-x
Authors: Velasco S, Kedaigle AJ, Simmons SK, Nash A, Rober M, Cayber B, Park J, Sber AJ, Villalobos E, Rogber CJ, Gilhool K, Esteves A, Chandrasekaran S, Pfister R, Lehtinen MK, Regev A, Arlotta P Duration: 180 days Cell source: Human iPSC / Human ESC
Abstract
Velasco et al. addressed the critical reproducibility challenge in brain organoid research by developing an optimized guided differentiation protocol that generates cortical organoids with consistent cell type diversity across individual organoids, batches, and iPSC lines. Single-cell RNA-sequencing over a 6-month culture period confirmed the reliable generation of all major cortical neuron subtypes.
Cell source
Human iPSC / Human ESC
Application
Reproducible Cortical Organoid Production
No step-by-step SOP yet
OrganMatch™ lists this published method, but there is no step-by-step SOP for it yet.
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