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BRAIN Publication-derived
Hindbrain 2D Cell Cultures for Developmental Studies
No SOP yet
Source Muguruma et al. · RIKEN Center for Developmental Biology, Kobe, Japan
Authors: Muguruma K, Nishiyama A, Kawakami H, Hashimoto K, Sasai Y Duration: 70 days Cell source: Human ESC
Abstract
Muguruma et al. developed a protocol for generating cerebellar organoids from human ESCs using FGF2, FGF19, and SDF1 to recapitulate isthmus organizer signaling and cerebellar plate formation. The organoids contained both Purkinje cell precursors and granule cell progenitors with layered organization, providing the first model for studying cerebellar development and ataxia disorders.
Cell source
Human ESC
Application
Cerebellar Development Studies
No step-by-step SOP yet
OrganMatch™ lists this published method, but there is no step-by-step SOP for it yet.
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