Self-Organization of Polarized Cerebellar Tissue from Human
Source Muguruma et al. · RIKEN Center for Developmental Biology, Kobe, Japan
Abstract
Muguruma et al. developed a protocol for generating cerebellar organoids from human ESCs using FGF2, FGF19, and SDF1 to recapitulate isthmus organizer signaling and cerebellar plate formation. The organoids contained both Purkinje cell precursors and granule cell progenitors with layered organization, providing the first model for studying cerebellar development and ataxia disorders.
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Attribution
This SOP was authored by Organthis based on the published method in Muguruma et al.. The originating laboratory holds no rights in this SOP and has not endorsed it unless marked Verified.
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