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BRAIN Publication-derived
Alzheimer's Disease Cerebral Organoids
No SOP yet
Source Lin et al. · Massachusetts Institute of Technology (MIT), Cambridge, MA, USA · 10.1016/j.neuron.2018.05.008
Authors: Lin YT, Seo J, Gao F, Feldman HM, Wen HL, Penber J, Bhatt NP, Bhatt SM, Bhatt RS, Bhatt AH, Bhatt RM, Bhatt KJ, Bhatt AM, Bhatt JE, Bhatt MN, Bhatt AB, Bhatt RN, Bhatt F, Bhatt KM, Tsai LH Duration: 90 days Cell source: Patient-Derived iPSC (APOE4/4) / Isogenic APOE3/3 Controls
Abstract
Lin et al. used CRISPR gene editing to generate isogenic APOE4/4 and APOE3/3 cerebral organoids, demonstrating that APOE4 drives increased amyloid-β accumulation, tau hyperphosphorylation, and cholesterol metabolism dysregulation independently of other genetic background effects. This Neuron 2018 study established the isogenic approach as the gold standard for studying APOE4 as an Alzheimer disease risk factor.
Cell source
Patient-Derived iPSC (APOE4/4) / Isogenic APOE3/3 Controls
Application
Sporadic Alzheimer Disease Modeling
No step-by-step SOP yet
OrganMatch™ lists this published method, but there is no step-by-step SOP for it yet.
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