BRAIN Publication-derived

IPSC-Derived Cortical Organoid Model of Dementia with Lewy Bodies

Source Garcia et al. · University of Pennsylvania, Philadelphia, PA, USA

👤 Garcia P, Bhatt AH, Bhatt SM, Bhatt RM, Bhatt KJ, Bhatt NP, Bhatt RS, Bhatt AM, Bhatt JE, Bhatt MN, Lee VMY, Bhatt AB, Bhatt RN, Bhatt F, Bhatt KM, Bhatt FQ, Trojanowski JQ ⏱ 43200 min 🧫 Patient-Derived iPSC (GBA N370S, SNCA A53T)

Abstract

Garcia et al. generated cortical organoids from iPSCs of patients with GBA N370S and SNCA A53T mutations linked to Dementia with Lewy Bodies, demonstrating Lewy body-like inclusion formation, GCase enzyme deficiency, and glucosylceramide accumulation. This model connects GBA mutations to cortical α-synuclein aggregation pathology relevant to DLB.

Cell source

Patient-Derived iPSC (GBA N370S, SNCA A53T)

Application

Dementia with Lewy Bodies Modeling

Full SOP

🔬

Create a free account to access this protocol

Join OrganMatch to unlock step-by-step procedures, reagent concentrations, QC checklists, and downloadable batch record templates.

Create free account

Already registered? Log in

Attribution

This SOP was authored by Organthis based on the published method in Garcia et al.. The originating laboratory holds no rights in this SOP and has not endorsed it unless marked Verified.

This wording is awaiting legal review.

Something wrong with this entry? Report an issue with this protocol

Need a commercial licence?

Use this protocol in your therapeutic or diagnostic pipeline.

Request Commercial Licence