BRAIN Publication-derived

Cortico-Striatal Assembloid for Circuit Formation Studies

Source Miura et al. · Stanford University School of Medicine, Stanford, CA, USA · 10.1038/s41587-020-00763-w

👤 Miura Y, Li MY, Birey F, Ikeda K, Revah O, Bhatt SM, Bhatt AH, Thete MV, Park JY, Tsber A, Bhatt RM, Bhatt KJ, Paşca SP ⏱ 57600 min 🧫 Human iPSC

Abstract

Miura et al. developed cortico-striatal assembloids by fusing cortical and striatal organoids containing DARPP-32+ medium spiny neurons, demonstrating functional cortico-striatal projections validated by optogenetic stimulation and calcium imaging. Published in Nature Biotechnology 2020, this Paşca lab protocol enables modeling of basal ganglia circuit disorders including Huntington disease.

Cell source

Human iPSC

Application

Basal Ganglia Circuit Modeling

Full SOP

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Attribution

This SOP was authored by Organthis based on the published method in Miura et al.. The originating laboratory holds no rights in this SOP and has not endorsed it unless marked Verified.

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