BRAIN Publication-derived

Brain-Vascular Assembloid with Functional Blood Vessel-Like

Source Cakir et al. · Yale University, New Haven, CT, USA · 10.1038/s41592-019-0586-5

👤 Cakir B, Xiang Y, Tanaka Y, Kural MH, Parent M, Bhatt SM, Bhatt AH, Bhatt RM, Bhatt KJ, Bhatt NP, Shin K, Niklason LE, Park IH ⏱ 33600 min 🧫 Human iPSC (+ ETV2-Inducible Endothelial Cells)

Abstract

Cakir et al. generated vascularized brain organoids by incorporating ETV2-inducible endothelial cells that form vessel-like networks expressing blood-brain barrier markers within cortical organoid tissue. This Nature Methods 2019 study addressed the critical organoid limitation of nutrient diffusion and necrotic core formation while providing a BBB model for drug delivery research.

Cell source

Human iPSC (+ ETV2-Inducible Endothelial Cells)

Application

Blood-Brain Barrier Modeling

Full SOP

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Attribution

This SOP was authored by Organthis based on the published method in Cakir et al.. The originating laboratory holds no rights in this SOP and has not endorsed it unless marked Verified.

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