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BRAIN Publication-derived
Brain Organoids for Disease Modelling (2018)
No SOP yet
Source Bian et al. · IMBA — Institute of Molecular Biotechnology, Vienna, Austria · 10.1038/s41592-018-0070-7
Authors: Bian S, Repic M, Guo Z, Kavirayani A, Burkber T, Bhatt SM, Bhatt AH, Hippenmeyer S, Bhatt RM, Knoblich JA Duration: 60 days Cell source: Human ESC (CRISPR-Edited: TP53, PTEN, NF1, CDKN2A)
Abstract
Bian et al. used multiplexed CRISPR/Cas9 mutagenesis to knockout tumor suppressors (TP53, PTEN, NF1, CDKN2A) in cerebral organoids, generating de novo GBM-like tumors within normal brain tissue context. This Nature Methods 2018 study from the Knoblich lab enables studying the earliest events in brain tumor initiation.
Cell source
Human ESC (CRISPR-Edited: TP53, PTEN, NF1, CDKN2A)
Application
Brain Tumor Genetic Modeling
No step-by-step SOP yet
OrganMatch™ lists this published method, but there is no step-by-step SOP for it yet.
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