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BRAIN Publication-derived

CRISPR Screen in Brain Organoids

Source Bian et al. · IMBA — Institute of Molecular Biotechnology, Vienna, Austria · 10.1038/s41592-018-0070-7

👤 Bian S, Repic M, Guo Z, Kavirayani A, Burkber T, Bhatt SM, Bhatt AH, Hippenmeyer S, Bhatt RM, Knoblich JA ⏱ 28800 min 🧫 Human ESC (CRISPR-Edited: TP53, PTEN, NF1, CDKN2A)

Abstract

Bian et al. used multiplexed CRISPR/Cas9 mutagenesis to knockout tumor suppressors (TP53, PTEN, NF1, CDKN2A) in cerebral organoids, generating de novo GBM-like tumors within normal brain tissue context. This Nature Methods 2018 study from the Knoblich lab enables studying the earliest events in brain tumor initiation.

Cell source
Human ESC (CRISPR-Edited: TP53, PTEN, NF1, CDKN2A)
Application
Brain Tumor Genetic Modeling

Full SOP

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Attribution

This SOP was authored by Organthis based on the published method in Bian et al.. The originating laboratory holds no rights in this SOP and has not endorsed it unless marked Verified.

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