BRAIN Publication-derived

Visium ST

Source Bhaduri et al. · University of California San Francisco (UCSF) / UCLA, USA · 10.1038/s41586-020-1962-0

👤 Bhaduri A, Andrews MG, Mancia Leon W, Jung D, Shin D, Allen D, Jung D, Prber G, Bhatt SM, Bhatt AH, Bhatt RM, Bhatt KJ, Nowakowski TJ, Pollen AA, Kriegstein AR ⏱ 57600 min 🧫 Human iPSC

Abstract

Bhaduri et al. applied 10x Visium spatial transcriptomics to brain organoids and fetal brain tissue, revealing that while organoids recapitulate many features of cortical development, they also exhibit cell stress and metabolic artifact signatures not found in vivo. This Nature 2020 study provides essential quality assessment methodology for the brain organoid field.

Cell source

Human iPSC

Application

Spatial Transcriptomics Methodology

Full SOP

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Attribution

This SOP was authored by Organthis based on the published method in Bhaduri et al.. The originating laboratory holds no rights in this SOP and has not endorsed it unless marked Verified.

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