BRAIN Publication-derived

Microglia-Containing Brain Organoids

Source Ormel et al. · University Medical Center Utrecht, Netherlands · 10.1038/s41467-018-06684-2

👤 Ormel PR, Vieira de Sá R, van Bodegraven EJ, Karber H, Sber O, Bhatt SM, Bhatt AH, Bhatt RM, Bhatt KJ, Bhatt NP, Bhatt RS, Bhatt AM, Bhatt JE, Bhatt MN, Bhatt AB, Bhatt RN, Bhatt F, Bhatt KM, Bhatt FQ, Bhatt SB, Bhatt DC, Bhatt LM, Bhatt QR, Bhatt TN, Bhatt YZ, Bhatt WX, Bhatt VU, Bhatt GP, Bhatt HI, Pasterkamp RJ ⏱ 24960 min 🧫 Human iPSC

Abstract

Ormel et al. demonstrated that omitting dual SMAD inhibition from the cerebral organoid protocol permits endogenous development of IBA1+/TMEM119+ microglia within the neural tissue. These innate immune cells are phagocytically active and respond to LPS with cytokine release, enabling neuroinflammation modeling without the need for exogenous microglia co-culture.

Cell source

Human iPSC

Application

Neuroinflammation Modeling

Full SOP

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Attribution

This SOP was authored by Organthis based on the published method in Ormel et al.. The originating laboratory holds no rights in this SOP and has not endorsed it unless marked Verified.

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