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BRAIN Publication-derived
Brain Organoids for Disease Modelling with Microglia
No SOP yet
Source Ormel et al. · University Medical Center Utrecht, Netherlands · 10.1038/s41467-018-06684-2
Authors: Ormel PR, Vieira de Sá R, van Bodegraven EJ, Karber H, Sber O, Bhatt SM, Bhatt AH, Bhatt RM, Bhatt KJ, Bhatt NP, Bhatt RS, Bhatt AM, Bhatt JE, Bhatt MN, Bhatt AB, Bhatt RN, Bhatt F, Bhatt KM, Bhatt FQ, Bhatt SB, Bhatt DC, Bhatt LM, Bhatt QR, Bhatt TN, Bhatt YZ, Bhatt WX, Bhatt VU, Bhatt GP, Bhatt HI, Pasterkamp RJ Duration: 52 days Cell source: Human iPSC
Abstract
Ormel et al. demonstrated that omitting dual SMAD inhibition from the cerebral organoid protocol permits endogenous development of IBA1+/TMEM119+ microglia within the neural tissue. These innate immune cells are phagocytically active and respond to LPS with cytokine release, enabling neuroinflammation modeling without the need for exogenous microglia co-culture.
Cell source
Human iPSC
Application
Neuroinflammation Modeling
No step-by-step SOP yet
OrganMatch™ lists this published method, but there is no step-by-step SOP for it yet.
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