Back to browse
BRAIN Publication-derived
Retinal Organoids for Disease Modelling with Gene Editing
No SOP yet
Source Lane et al. · Moorfields Eye Hospital / UCL Institute of Ophthalmology, London, UK · 10.1016/j.stemcr.2020.05.018
Authors: Lane A, Jovanovic K, Shortall C, Ottaviani D, Panes AB, Schwarz N, Guarber R, Hims MM, Ripamonti C, Munro PM, Fitzpatrick P, Henderson RH, Cheetham ME, Hardcastle AJ Duration: 180 days Cell source: Patient-Derived iPSC (X-linked RP) + CRISPR-Corrected Isogenic
Abstract
Lane et al. combined CRISPR/Cas9 gene correction of RPGR mutations in patient-derived iPSCs with retinal organoid differentiation, creating isogenic pairs that revealed ciliary localization defects as the primary pathogenic mechanism in X-linked retinitis pigmentosa.
Cell source
Patient-Derived iPSC (X-linked RP) + CRISPR-Corrected Isogenic
Application
X-linked RP Disease Modeling
No step-by-step SOP yet
OrganMatch™ lists this published method, but there is no step-by-step SOP for it yet.
Something wrong with this entry? Report an issue with this protocol