BRAIN Publication-derived

CRISPR-Corrected RPGR Retinal Organoids for X-Linked RP

Source Lane et al. · Moorfields Eye Hospital / UCL Institute of Ophthalmology, London, UK · 10.1016/j.stemcr.2020.05.018

👤 Lane A, Jovanovic K, Shortall C, Ottaviani D, Panes AB, Schwarz N, Guarber R, Hims MM, Ripamonti C, Munro PM, Fitzpatrick P, Henderson RH, Cheetham ME, Hardcastle AJ ⏱ 86400 min 🧫 Patient-Derived iPSC (X-linked RP) + CRISPR-Corrected Isogenic

Abstract

Lane et al. combined CRISPR/Cas9 gene correction of RPGR mutations in patient-derived iPSCs with retinal organoid differentiation, creating isogenic pairs that revealed ciliary localization defects as the primary pathogenic mechanism in X-linked retinitis pigmentosa.

Cell source

Patient-Derived iPSC (X-linked RP) + CRISPR-Corrected Isogenic

Application

X-linked RP Disease Modeling

Full SOP

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Attribution

This SOP was authored by Organthis based on the published method in Lane et al.. The originating laboratory holds no rights in this SOP and has not endorsed it unless marked Verified.

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