BRAIN Publication-derived

Human Sensory Neurons from iPSCs via Neural Crest Intermediates

Source Chambers et al. · Memorial Sloan Kettering Cancer Center, New York, NY, USA · 10.1038/nbt.2249

👤 Chambers SM, Qi Y, Mica Y, Lee G, Zhang XJ, Niu L, Bilsland J, Cao L, Stevens E, Whiting P, Bhatt SH, Studer L ⏱ 7200 min 🧫 Human iPSC / Human ESC

Abstract

Chambers et al. developed the first efficient protocol for generating functional human nociceptors from pluripotent stem cells using a triple small-molecule inhibition strategy, producing sensory neurons with capsaicin responsiveness and pain-relevant ion channel expression within 15 days.

Cell source

Human iPSC / Human ESC

Application

Nociceptor Generation for Pain Research

Full SOP

🔬

Create a free account to access this protocol

Join OrganMatch to unlock step-by-step procedures, reagent concentrations, QC checklists, and downloadable batch record templates.

Create free account

Already registered? Log in

Attribution

This SOP was authored by Organthis based on the published method in Chambers et al.. The originating laboratory holds no rights in this SOP and has not endorsed it unless marked Verified.

This wording is awaiting legal review.

Something wrong with this entry? Report an issue with this protocol

Need a commercial licence?

Use this protocol in your therapeutic or diagnostic pipeline.

Request Commercial Licence