Gastric Organoid Culture Media Preparation
Source <UNKNOWN>
Abstract
This protocol describes the preparation of optimized culture media for gastric organoid differentiation and maintenance. The media formulation incorporates basal Advanced DMEM/F12 supplemented with growth factors (R-spondin 1, Noggin, EGF), hormones (Gastrin, Prostaglandin E2), signaling modulators (A-83-01, SB202190), and supportive components (N2, B27, Glutamax, HEPES) to support sustained gastric epithelial organoid development.
Protocol overview
19 steps across 7 phases
- 1 Prepare Advanced DMEM/F12 base
- 2 Add HEPES buffer
- 3 Add Glutamax supplement
- 4 Add antibiotic protection
- 5 Add N2 supplement
- 6 Add B27 supplement
- 7 Prepare and add R-spondin 1
- 8 Prepare and add Noggin
- 9 Prepare and add EGF
- 10 Add n-Acetylcysteine
- 11 Add Niacinamide
- 12 Prepare and add A-83-01
- 13 Prepare and add SB202190
- 14 Prepare and add Gastrin
- 15 Prepare and add Prostaglandin E2
- 16 Mix media thoroughly
- 17 Filter-sterilize the media
- 18 Check media pH
- 19 Store the media
Full SOP
Create a free account to access this protocol
Join OrganMatch to unlock step-by-step procedures, reagent concentrations, QC checklists, and downloadable batch record templates.
Create free accountAlready registered? Log in
Attribution
This SOP was authored by Organthis based on the published method in the published method. The originating laboratory holds no rights in this SOP and has not endorsed it unless marked Verified.
This wording is awaiting legal review.
Something wrong with this entry? Report an issue with this protocol