Skip to content
Back to browse
LIVER Publication-derived

Liver Organoids for Drug Screening and Disease Modelling

Full SOP with Academic or a plan

Source Mun et al., 2019 · Korea Research Institute of Bioscience and Biotechnology (KRIBB) · 10.1016/j.jhep.2019.06.030

Authors: Seon Ju Mun, Jae-Sung Ryu, Mi-Ok Lee, Ye Seul Son, Soo Jin Oh, Hyun-Soo Cho, Mi-Young Son, Dae-Soo Kim, Su Jung Kim, Hyun Ju Yoo, Ho-Joon Lee, Janghwan Kim, Cho-Rok Jung, Kyung-Sook Chung, Myung Jin Son Duration: 25 days 6 phases Cell source: Human iPSC, Human ESC

Abstract

This protocol describes the generation of expandable, functionally mature human hepatocyte-like liver organoids from pluripotent stem cells (iPSCs and hESCs). The organoids exhibit self-renewal capacity over long-term culture (>24 passages), maintain mature hepatic functions including drug metabolism and serum protein production, and can be used for disease modeling (hepatic steatosis), drug toxicity prediction, and drug efficacy screening.

Cell source
Human iPSC, Human ESC
Application
Disease modeling, Drug screening, Toxicity prediction

Protocol overview

16 steps across 6 phases

Phase 1 · Definitive Endoderm Differentiation Day 0-3
  1. a Seed PSCs on Matrigel-coated dishes
  2. b Exchange medium to definitive endoderm induction medium
  3. c Culture in definitive endoderm medium
Phase 2 · Hepatic Endoderm Differentiation Day 9-13
  1. a Exchange medium to hepatic endoderm induction medium
  2. b Culture under hypoxia
Phase 3 · Hepatic Maturation Day 13-25
  1. a Exchange medium to hepatic maturation medium
  2. b Culture under hypoxia
  3. c Continue culture under normoxia
Phase 4 · 3D Organoid Formation and Embedding Day 22-25
  1. a Collect floating cysts
  2. b Embed organoids in Matrigel dome
Phase 5 · Organoid Maintenance and Routine Culture Day 25 onwards
  1. a Routine medium replacement
  2. b Mechanical passaging
  3. c Alternative: Chemical passaging
  4. d Cryopreservation
Phase 6 · Optional: Further Differentiation Protocol (Expansion and Differentiation Media) Variable (2-3 days EM, then 6 days DM)
  1. a Switch to expansion medium
  2. b Switch to differentiation medium

Full SOP

Full SOPs need an account and a plan

Create an account, then get free Academic access (for verified academics) or choose a plan. Plans include step-by-step procedures, reagent concentrations, QC checklists and batch record templates.

Create an account

Already registered? Log in

Attribution

This SOP was written by Organthis based on the published method in Mun et al., 2019. Once the originating laboratory has confirmed it, the SOP is marked Verified.

Something wrong with this entry? Report an issue with this protocol

Need a commercial licence?
Use this protocol in your therapeutic or diagnostic pipeline.
Request Commercial Licence