CARDIAC Publication-derived

Free Cardiomyocyte Generation

Source Burridge et al. · Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, USA · 10.1038/nmeth.2999

👤 Burridge PW, Matsa E, Shukla P, Lin ZC, Churko JM, Ebert AD, Lan F, Diecke S, Huber B, Mordwinkin NM, Plews JR, Abilez OJ, Cui B, Gold JD, Wu JC ⏱ 6720 min 🧫 iPSC-Derived (Human iPSCs)

Abstract

Burridge et al. developed the first fully chemically defined, xeno-free protocol for iPSC-cardiomyocyte differentiation using CDM3 medium. The protocol eliminates all animal-derived components, achieves >95% purity with metabolic selection, and is directly compatible with GMP manufacturing for clinical translation.

Cell source

iPSC-Derived (Human iPSCs)

Application

Clinical-Grade CM Production

Full SOP

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Attribution

This SOP was authored by Organthis based on the published method in Burridge et al.. The originating laboratory holds no rights in this SOP and has not endorsed it unless marked Verified.

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