CARDIAC Publication-derived

Metabolic Selection for Cardiomyocyte Purification

Source Tohyama et al. · Department of Cardiology, Keio University School of Medicine, Tokyo, Japan

👤 Tohyama S, Hattori F, Sano M, Hishiki T, Nagahata Y, Matsuura T, Hashimoto H, Suzuki T, Yamashita H, Satoh Y, Egashira T, Seki T, Muraoka N, Yamakawa H, Ohgino Y, Tanaka T, Yoichi M, Yuasa S, Murata M, Suematsu M, Fukuda K ⏱ 12000 min 🧫 iPSC-Derived (Human iPSCs/ESCs)

Abstract

Tohyama et al. discovered that cardiomyocytes uniquely metabolize lactate under glucose-free conditions, enabling a simple metabolic selection strategy that achieves >99% cardiomyocyte purity. This non-genetic purification method has become a standard tool for generating transplantation-grade cardiomyocytes for both research and clinical applications.

Cell source

iPSC-Derived (Human iPSCs/ESCs)

Application

Cardiomyocyte Purification

Full SOP

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Attribution

This SOP was authored by Organthis based on the published method in Tohyama et al.. The originating laboratory holds no rights in this SOP and has not endorsed it unless marked Verified.

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