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CARDIAC Publication-derived
Cardiac 2D Cell Cultures for Regenerative Medicine (2013)
No SOP yet
Source Tohyama et al. · Department of Cardiology, Keio University School of Medicine, Tokyo, Japan
Authors: Tohyama S, Hattori F, Sano M, Hishiki T, Nagahata Y, Matsuura T, Hashimoto H, Suzuki T, Yamashita H, Satoh Y, Egashira T, Seki T, Muraoka N, Yamakawa H, Ohgino Y, Tanaka T, Yoichi M, Yuasa S, Murata M, Suematsu M, Fukuda K Duration: 25 days Cell source: iPSC-Derived (Human iPSCs/ESCs)
Abstract
Tohyama et al. discovered that cardiomyocytes uniquely metabolize lactate under glucose-free conditions, enabling a simple metabolic selection strategy that achieves >99% cardiomyocyte purity. This non-genetic purification method has become a standard tool for generating transplantation-grade cardiomyocytes for both research and clinical applications.
Cell source
iPSC-Derived (Human iPSCs/ESCs)
Application
Cardiomyocyte Purification
No step-by-step SOP yet
OrganMatch™ lists this published method, but there is no step-by-step SOP for it yet.
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