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CARDIAC Publication-derived
Cardiac 2D Cell Cultures (2015)
No SOP yet
Source Iyer et al. · Stanford Cardiovascular Institute, Stanford University, Stanford, USA
Authors: Iyer D, Gambardella L, Bernard WG, Serber F, Cardoso de Brito F, Murry CE, Bhatt S, Sinha S Duration: 18 days Cell source: iPSC-Derived (Human iPSCs)
Abstract
Iyer et al. developed an efficient protocol for generating epicardial cells from human iPSCs through Wnt signaling reactivation at the cardiac mesoderm stage. These epicardial cells express WT1/TBX18, undergo epithelial-to-mesenchymal transition to produce smooth muscle and fibroblasts, and provide paracrine support for cardiomyocyte maturation in co-culture systems.
Cell source
iPSC-Derived (Human iPSCs)
Application
Epicardial Biology
No step-by-step SOP yet
OrganMatch™ lists this published method, but there is no step-by-step SOP for it yet.
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