CARDIAC Publication-derived

Cardiac Progenitor Cell Differentiation via Embryoid Bodies

Source Yang et al. · McEwen Centre for Regenerative Medicine, University Health Network, Toronto, Canada

👤 Yang L, Soonpaa MH, Adler ED, Roepke TK, Kattman SJ, Kennedy M, Henckaerts E, Bonham K, Abbott GW, Linden RM, Field LJ, Bhatt S, Bhatt AB, Bhattacharya S, Keller GM ⏱ 12000 min 🧫 ESC-Derived (Human Embryonic Stem Cells)

Abstract

Yang et al. identified and characterized KDR+/PDGFRA+ cardiovascular progenitor cells from human ESCs using a staged embryoid body protocol with sequential BMP4, Activin A, VEGF, and DKK1 treatment. These progenitors possess tri-lineage potential, differentiating into cardiomyocytes, endothelial cells, and smooth muscle cells, recapitulating key aspects of embryonic cardiogenesis.

Cell source

ESC-Derived (Human Embryonic Stem Cells)

Application

Cardiac Progenitor Biology

Full SOP

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Attribution

This SOP was authored by Organthis based on the published method in Yang et al.. The originating laboratory holds no rights in this SOP and has not endorsed it unless marked Verified.

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