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CARDIAC Publication-derived
Ventricular 2D Cell Cultures for Developmental Studies
No SOP yet
Source Mills et al. · QIMR Berghofer Medical Research Institute, Brisbane, Australia · 10.1073/pnas.1702914114
Authors: Mills RJ, Titmarsh DM, Koenig X, Parker BL, Ryall JG, Quaife-Ryan GA, Voges HK, Hodge MP, Ferguson C, Drowley L, Plober AT, Needham EJ, Li QY, Porrello ER, Delbridge LM, Thomas A, Gregorevic P, Parton RG, Li LI, Hawkins SJ, Hudson JE Duration: 28 days Cell source: iPSC-Derived (Human iPSCs)
Abstract
Mills et al. developed human cardiac organoids (hCOs) that achieve structural and metabolic maturation through a fatty acid metabolic switching protocol. The resulting compact ventricular muscle organoids form T-tubules, exhibit mature calcium handling, and show positive force-frequency relationships, providing a more physiologically relevant platform for drug screening and disease modeling.
Cell source
iPSC-Derived (Human iPSCs)
Application
Cardiac Maturation Studies
No step-by-step SOP yet
OrganMatch™ lists this published method, but there is no step-by-step SOP for it yet.
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