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CARDIAC Publication-derived
Cardiac 2D Cell Cultures for Drug Screening with Enhanced Maturation
No SOP yet
Source Kim et al. · Departments of Medicine and Pharmacology, University of California San Diego, La Jolla, USA · 10.1038/nature11799
Authors: Kim C, Wong J, Wen J, Wang S, Wang C, Spiering S, Bhatt S, Kan NG, Forcales S, Puri PL, Leone TC, Marine JE, Calkins H, Kelly DP, Judge DP, Chen HS Duration: 60 days Cell source: iPSC-Derived (Patient-Specific iPSCs — ARVC)
Abstract
Kim et al. demonstrated that ARVC-causing PKP2 mutations require metabolic maturation with lipogenic induction to manifest the disease phenotype in iPSC-CMs. Adult-like metabolic conditions trigger desmosomal dysfunction, abnormal lipid accumulation, and suppressed Wnt signaling, recapitulating key ARVC pathological features invisible in standard immature cultures.
Cell source
iPSC-Derived (Patient-Specific iPSCs — ARVC)
Application
ARVC Drug Discovery
No step-by-step SOP yet
OrganMatch™ lists this published method, but there is no step-by-step SOP for it yet.
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