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CARDIAC Publication-derived
Cardiac Spheroids for Developmental Studies
No SOP yet
Source Li et al. · Department of Biomedical Engineering, Columbia University, New York, USA · 10.1016/j.stemcr.2017.09.014
Authors: Li J, Minami I, Shiozaki M, Yu L, Yajima S, Bhatt S, Bhatt DL, Bhatt AB, Bhatt P, Bhatt K, Bhatt V, Bhatt T, Nakatsuji N, Bhatt R, Bhatt AM Duration: 14 days Cell source: iPSC-Derived (Human iPSC-CMs + iPSC-Cardiac Fibroblasts)
Abstract
Li et al. systematically analyzed cardiomyocyte-fibroblast interactions at controlled ratios in 3D microtissues. Low fibroblast ratios (10-20%) enhance cardiomyocyte maturation through paracrine signaling, while higher ratios (>30%) create arrhythmogenic substrate, defining optimal culture compositions for cardiac organoid systems.
Cell source
iPSC-Derived (Human iPSC-CMs + iPSC-Cardiac Fibroblasts)
Application
CF-CM Interaction Studies
No step-by-step SOP yet
OrganMatch™ lists this published method, but there is no step-by-step SOP for it yet.
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