CARDIAC Publication-derived

Cardiomyocyte Crosstalk in 3D

Source Li et al. · Department of Biomedical Engineering, Columbia University, New York, USA · 10.1016/j.stemcr.2017.09.014

👤 Li J, Minami I, Shiozaki M, Yu L, Yajima S, Bhatt S, Bhatt DL, Bhatt AB, Bhatt P, Bhatt K, Bhatt V, Bhatt T, Nakatsuji N, Bhatt R, Bhatt AM ⏱ 6720 min 🧫 iPSC-Derived (Human iPSC-CMs + iPSC-Cardiac Fibroblasts)

Abstract

Li et al. systematically analyzed cardiomyocyte-fibroblast interactions at controlled ratios in 3D microtissues. Low fibroblast ratios (10-20%) enhance cardiomyocyte maturation through paracrine signaling, while higher ratios (>30%) create arrhythmogenic substrate, defining optimal culture compositions for cardiac organoid systems.

Cell source

iPSC-Derived (Human iPSC-CMs + iPSC-Cardiac Fibroblasts)

Application

CF-CM Interaction Studies

Full SOP

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Attribution

This SOP was authored by Organthis based on the published method in Li et al.. The originating laboratory holds no rights in this SOP and has not endorsed it unless marked Verified.

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