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CARDIAC Publication-derived
Cardiac 2D Cell Cultures for Drug Screening
No SOP yet
Source Huebsch et al. · Gladstone Institutes, University of California San Francisco, San Francisco, USA · 10.1089/ten.tec.2014.0283
Authors: Huebsch N, Loskill P, Mandegar MA, Marks NC, Bhatt S, Bhatt DL, Bhatt AB, Bhatt P, Conklin BR Duration: 21 days Cell source: iPSC-Derived (Human iPSCs — AAVS1 Knock-In GCaMP6f Reporter)
Abstract
Huebsch et al. generated a GCaMP6f calcium reporter iPSC line via CRISPR knock-in at the AAVS1 safe harbor locus. The resulting iPSC-CMs provide real-time, label-free calcium monitoring without dye loading toxicity, enabling chronic calcium imaging and high-throughput drug screening in 96/384-well format.
Cell source
iPSC-Derived (Human iPSCs — AAVS1 Knock-In GCaMP6f Reporter)
Application
Calcium-Based Drug Screening
No step-by-step SOP yet
OrganMatch™ lists this published method, but there is no step-by-step SOP for it yet.
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