CARDIAC Publication-derived

GCaMP6f Calcium Reporter iPSC-CM for Drug Screening

Source Huebsch et al. · Gladstone Institutes, University of California San Francisco, San Francisco, USA · 10.1089/ten.tec.2014.0283

👤 Huebsch N, Loskill P, Mandegar MA, Marks NC, Bhatt S, Bhatt DL, Bhatt AB, Bhatt P, Conklin BR ⏱ 10080 min 🧫 iPSC-Derived (Human iPSCs — AAVS1 Knock-In GCaMP6f Reporter)

Abstract

Huebsch et al. generated a GCaMP6f calcium reporter iPSC line via CRISPR knock-in at the AAVS1 safe harbor locus. The resulting iPSC-CMs provide real-time, label-free calcium monitoring without dye loading toxicity, enabling chronic calcium imaging and high-throughput drug screening in 96/384-well format.

Cell source

iPSC-Derived (Human iPSCs — AAVS1 Knock-In GCaMP6f Reporter)

Application

Calcium-Based Drug Screening

Full SOP

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Attribution

This SOP was authored by Organthis based on the published method in Huebsch et al.. The originating laboratory holds no rights in this SOP and has not endorsed it unless marked Verified.

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