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CARDIAC Publication-derived
Cardiac 2D Cell Cultures for Developmental Studies with Enhanced Maturation
No SOP yet
Source Ronaldson-Bouchard et al. · Department of Biomedical Engineering, Columbia University, New York, USA · 10.1038/s41586-018-0016-3
Authors: Ronaldson-Bouchard K, Ma SP, Yeager K, Chen T, Song L, Sirabella D, Morikawa K, Teles D, Yazawa M, Vunjak-Novakovic G Duration: 28 days Cell source: iPSC-Derived (Human iPSC-CMs)
Abstract
Ronaldson-Bouchard et al. achieved adult-like cardiac tissue maturation through intensity training — progressive electrical stimulation ramping from 0.33 to 6 Hz over two weeks. The resulting tissues develop T-tubules, adult-like gene expression, conduction velocity exceeding 25 cm/s, and positive force-frequency relationships, representing the most mature human cardiac tissue in vitro.
Cell source
iPSC-Derived (Human iPSC-CMs)
Application
Cardiac Tissue Maturation
No step-by-step SOP yet
OrganMatch™ lists this published method, but there is no step-by-step SOP for it yet.
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