CARDIAC Publication-derived

Cardiac Conduction Velocity Maturation by Electrical Training

Source Ronaldson-Bouchard et al. · Department of Biomedical Engineering, Columbia University, New York, USA · 10.1038/s41586-018-0016-3

👤 Ronaldson-Bouchard K, Ma SP, Yeager K, Chen T, Song L, Sirabella D, Morikawa K, Teles D, Yazawa M, Vunjak-Novakovic G ⏱ 13440 min 🧫 iPSC-Derived (Human iPSC-CMs)

Abstract

Ronaldson-Bouchard et al. achieved adult-like cardiac tissue maturation through intensity training — progressive electrical stimulation ramping from 0.33 to 6 Hz over two weeks. The resulting tissues develop T-tubules, adult-like gene expression, conduction velocity exceeding 25 cm/s, and positive force-frequency relationships, representing the most mature human cardiac tissue in vitro.

Cell source

iPSC-Derived (Human iPSC-CMs)

Application

Cardiac Tissue Maturation

Full SOP

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Attribution

This SOP was authored by Organthis based on the published method in Ronaldson-Bouchard et al.. The originating laboratory holds no rights in this SOP and has not endorsed it unless marked Verified.

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