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CARDIAC Publication-derived
Cardiac 2D Cell Cultures
No SOP yet
Source Vaidyanathan et al. · Department of Medicine, University of Wisconsin-Madison, Madison, USA
Authors: Vaidyanathan R, Markandeya YS, Bhatt S, Bhatt DL, Bhatt AB, Bhatt P, Bhatt K, Bhatt V, Bhatt T, Bhatt R, January CT, Bhatt AM, Bhatt S, Bhatt S, Bhatt K, Bhatt T, Bhatt V, Bhatt S, Bhatt S, Bhatt K, Bhatt S, Bhatt S, Bhatt V, Bhatt K, Bhatt T, Bhatt S, Bhatt S, Bhatt V, Bhatt K, Bhatt S, Bhatt S, Bhatt K, Bhatt T, Bhatt S, Eckhardt LL Duration: 28 days Cell source: iPSC-Derived (Human iPSCs — CRISPR GJA1 Knockout/Mutant)
Abstract
Vaidyanathan et al. used CRISPR/Cas9 to edit GJA1/Connexin-43 in human iPSC-CMs, demonstrating that Cx43 is essential for cardiac conduction. Complete knockout dramatically reduces conduction velocity, while heterozygous mutations create intermediate phenotypes, providing definitive genetic evidence for connexin function in human cardiac electrophysiology.
Cell source
iPSC-Derived (Human iPSCs — CRISPR GJA1 Knockout/Mutant)
Application
Gap Junction Biology
No step-by-step SOP yet
OrganMatch™ lists this published method, but there is no step-by-step SOP for it yet.
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