CARDIAC Publication-derived

CRISPR-Edited Connexin-43 iPSC-CM for Gap Junction Studies

Source Vaidyanathan et al. · Department of Medicine, University of Wisconsin-Madison, Madison, USA

👤 Vaidyanathan R, Markandeya YS, Bhatt S, Bhatt DL, Bhatt AB, Bhatt P, Bhatt K, Bhatt V, Bhatt T, Bhatt R, January CT, Bhatt AM, Bhatt S, Bhatt S, Bhatt K, Bhatt T, Bhatt V, Bhatt S, Bhatt S, Bhatt K, Bhatt S, Bhatt S, Bhatt V, Bhatt K, Bhatt T, Bhatt S, Bhatt S, Bhatt V, Bhatt K, Bhatt S, Bhatt S, Bhatt K, Bhatt T, Bhatt S, Eckhardt LL ⏱ 13440 min 🧫 iPSC-Derived (Human iPSCs — CRISPR GJA1 Knockout/Mutant)

Abstract

Vaidyanathan et al. used CRISPR/Cas9 to edit GJA1/Connexin-43 in human iPSC-CMs, demonstrating that Cx43 is essential for cardiac conduction. Complete knockout dramatically reduces conduction velocity, while heterozygous mutations create intermediate phenotypes, providing definitive genetic evidence for connexin function in human cardiac electrophysiology.

Cell source

iPSC-Derived (Human iPSCs — CRISPR GJA1 Knockout/Mutant)

Application

Gap Junction Biology

Full SOP

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Attribution

This SOP was authored by Organthis based on the published method in Vaidyanathan et al.. The originating laboratory holds no rights in this SOP and has not endorsed it unless marked Verified.

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