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CARDIAC Publication-derived
Cardiac 2D Cell Cultures for Developmental Studies (2019)
No SOP yet
Source Yang et al. · Department of Medicine, Stanford Cardiovascular Institute, Stanford University, Stanford, USA · 10.1016/j.stemcr.2019.08.014
Authors: Yang X, Rodriguez ML, Leonard A, Sun L, Fischer KA, Wang Y, Ritterhoff J, Zhao L, Kolwicz SC, Pabon L, Reinecke H, Sniadecki NJ, Tian R, Ruohola-Baker H, Xu H, Murry CE Duration: 30 days Cell source: iPSC-Derived (Human iPSCs)
Abstract
Yang et al. developed a fatty acid-based metabolic maturation protocol replacing glucose with galactose and supplementing palmitate/oleate to drive the glycolysis-to-FAO switch in iPSC-CMs. The resulting cells show adult-like metabolic profiles, improved sarcomere organization, and MYH7/MYH6 isoform switching, representing a widely adopted maturation strategy.
Cell source
iPSC-Derived (Human iPSCs)
Application
Metabolic Maturation
No step-by-step SOP yet
OrganMatch™ lists this published method, but there is no step-by-step SOP for it yet.
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