CARDIAC Publication-derived

CMs (iPSC)

Source Yang et al. · Department of Medicine, Stanford Cardiovascular Institute, Stanford University, Stanford, USA · 10.1016/j.stemcr.2019.08.014

👤 Yang X, Rodriguez ML, Leonard A, Sun L, Fischer KA, Wang Y, Ritterhoff J, Zhao L, Kolwicz SC, Pabon L, Reinecke H, Sniadecki NJ, Tian R, Ruohola-Baker H, Xu H, Murry CE ⏱ 14400 min 🧫 iPSC-Derived (Human iPSCs)

Abstract

Yang et al. developed a fatty acid-based metabolic maturation protocol replacing glucose with galactose and supplementing palmitate/oleate to drive the glycolysis-to-FAO switch in iPSC-CMs. The resulting cells show adult-like metabolic profiles, improved sarcomere organization, and MYH7/MYH6 isoform switching, representing a widely adopted maturation strategy.

Cell source

iPSC-Derived (Human iPSCs)

Application

Metabolic Maturation

Full SOP

🔬

Create a free account to access this protocol

Join OrganMatch to unlock step-by-step procedures, reagent concentrations, QC checklists, and downloadable batch record templates.

Create free account

Already registered? Log in

Attribution

This SOP was authored by Organthis based on the published method in Yang et al.. The originating laboratory holds no rights in this SOP and has not endorsed it unless marked Verified.

This wording is awaiting legal review.

Something wrong with this entry? Report an issue with this protocol

Need a commercial licence?

Use this protocol in your therapeutic or diagnostic pipeline.

Request Commercial Licence