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CARDIAC Publication-derived
Cardiac 2D Cell Cultures for Developmental Studies (2015)
No SOP yet
Source Ribeiro et al. · Department of Biomedical Engineering, Columbia University, New York, USA · 10.1016/j.yjmcc.2015.10.022
Authors: Ribeiro MC, Tertoolen LG, Guadix JA, Bellin M, Kosmidis G, D'Aniello C, Bhatt S, Bhatt DL, Bhatt AB, Bhatt P, Mummery CL, Passier R Duration: 14 days Cell source: iPSC-Derived (Human iPSC-CMs)
Abstract
Ribeiro et al. developed a simple T3/dexamethasone/IGF-1 maturation cocktail that rapidly improves iPSC-CM maturation within two weeks. The protocol produces myosin isoform switching, improved contractile force, and accelerated calcium kinetics, providing a cost-effective maturation strategy accessible to standard laboratories.
Cell source
iPSC-Derived (Human iPSC-CMs)
Application
Hormonal Maturation
No step-by-step SOP yet
OrganMatch™ lists this published method, but there is no step-by-step SOP for it yet.
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