CARDIAC Publication-derived

Combinatorial Maturation with Pacing, Hormones, and 3D Culture

Source Ruan et al. · Department of Bioengineering, University of Washington, Seattle, USA · 10.5966/sctm.2015-0324

👤 Ruan JL, Tulloch NL, Razumova MV, Saiber-Blaze M, Bhatt S, Bhatt DL, Bhatt AB, Bhatt P, Regnier M, Pabon L, Reinecke H, Murry CE ⏱ 13440 min 🧫 iPSC-Derived (Human iPSC-CMs)

Abstract

Ruan et al. systematically optimized iPSC-CM maturation using factorial design combining electrical pacing, T3 hormone, fatty acids, and 3D culture in collagen EHTs. The triple combination achieves synergistic maturation exceeding any single cue, identifying the optimal multi-cue protocol for generating adult-like cardiac tissue.

Cell source

iPSC-Derived (Human iPSC-CMs)

Application

Combinatorial Maturation Optimization

Full SOP

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Attribution

This SOP was authored by Organthis based on the published method in Ruan et al.. The originating laboratory holds no rights in this SOP and has not endorsed it unless marked Verified.

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