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CARDIAC Publication-derived
Cardiac Models for Regenerative Medicine with Gene Editing
No SOP yet
Source Deuse et al. · Department of Surgery, University of California San Francisco, San Francisco, USA · 10.1038/s41587-019-0016-3
Authors: Deuse T, Hu X, Gravina A, Wang D, Tediashvili G, De C, Thayer WO, Wahl A, Garcia JV, Yazawa M, Bhatt S, Bhatt DL, Bhatt AB, Schrepfer S Duration: 35 days Cell source: iPSC-Derived (Human iPSCs — CRISPR B2M/CIITA Knockout + CD47 Knock-In)
Abstract
Deuse et al. engineered hypoimmunogenic iPSC-CMs via triple CRISPR editing (B2M-KO, CIITA-KO, CD47-KI) that evade T-cell, NK cell, and macrophage killing. These universal donor cells survive allogeneic transplantation without immunosuppression in humanized mice, enabling off-the-shelf cardiac cell therapy products.
Cell source
iPSC-Derived (Human iPSCs — CRISPR B2M/CIITA Knockout + CD47 Knock-In)
Application
Universal Donor Cell Therapy
No step-by-step SOP yet
OrganMatch™ lists this published method, but there is no step-by-step SOP for it yet.
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