Gastric Organoids for Disease Modelling (2015)
Source Huch et al., 2015 · Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge · 10.1242/dev.118570
Abstract
This protocol generates human gastric organoids (hGOs) through directed differentiation of human embryonic stem cells (hESCs) or human induced pluripotent stem cells (hiPSCs) into definitive endoderm, followed by stepwise induction of anterior foregut and antral stomach specification. The resulting three-dimensional organoids recapitulate gastric epithelial development in vitro and contain multiple gastric cell types including enteroendocrine cells, producing a physiologically relevant model of the human stomach antrum.
Protocol overview
7 steps across 3 phases
- a Seed hESCs or hiPSCs for differentiation
- b Treat with Activin A to induce definitive endoderm
- a Apply Wnt, FGF4, and noggin to promote foregut fate
- b Transfer to 3D extracellular matrix with retinoic acid
- c Culture in EGF-rich medium to generate mature gastric organoids
- a Maintain organoids in 3D Matrigel culture
- b Monitor organoid formation and cellular differentiation
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Attribution
This SOP was written by Organthis based on the published method in Huch et al., 2015. Once the originating laboratory has confirmed it, the SOP is marked Verified.
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