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INTESTINAL Publication-derived
Colonic Cancer Tumouroids
No SOP yet
Source Sato et al. · Hubrecht Institute, KNAW and University Medical Centre Utrecht, Utrecht, Netherlands
Authors: Sato T, Stange DE, Ferrante M, Vries RGJ, Van Es JH, Van den Brink S, Van Houdt WJ, Pronk A, Van Gorp J, Siersema PD, Clevers H Duration: 45 days Cell source: Primary (Human Surgical Tissue)
Abstract
Extended the Clevers organoid system to human tissues, establishing long-term culture of organoids from human colon, adenoma, adenocarcinoma, and Barrett's esophagus. Key additions of Wnt3A, nicotinamide, A83-01, and SB202190 enabled indefinite human organoid expansion, forming the basis for all subsequent human GI organoid work.
Cell source
Primary (Human Surgical Tissue)
Application
Human GI Disease Modeling
No step-by-step SOP yet
OrganMatch™ lists this published method, but there is no step-by-step SOP for it yet.
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