INTESTINAL Publication-derived

Dependent Niche

Source Ootani et al. · Stanford University School of Medicine, Stanford, USA · 10.1038/nm.1951

👤 Ootani A, Li X, Sangiorgi E, Ho QT, Ueno H, Toda S, Sugihara H, Fujimoto K, Weissman IL, Capecchi MR, Kuo CJ ⏱ 28800 min 🧫 Primary (Mouse and Human Intestinal Tissue)

Abstract

An alternative intestinal organoid culture system using collagen gel air-liquid interface (ALI) with stromal support, published concurrently with the Clevers Matrigel-based system. Achieved sustained culture for over 350 days with all differentiated epithelial lineages and mesenchymal compartment preservation.

Cell source

Primary (Mouse and Human Intestinal Tissue)

Application

Long-Term Intestinal Culture

Full SOP

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Attribution

This SOP was authored by Organthis based on the published method in Ootani et al.. The originating laboratory holds no rights in this SOP and has not endorsed it unless marked Verified.

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