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INTESTINAL Publication-derived
Intestinal 2D Cell Cultures
No SOP yet
Source Ootani et al. · Stanford University School of Medicine, Stanford, USA · 10.1038/nm.1951
Authors: Ootani A, Li X, Sangiorgi E, Ho QT, Ueno H, Toda S, Sugihara H, Fujimoto K, Weissman IL, Capecchi MR, Kuo CJ Duration: 60 days Cell source: Primary (Mouse and Human Intestinal Tissue)
Abstract
An alternative intestinal organoid culture system using collagen gel air-liquid interface (ALI) with stromal support, published concurrently with the Clevers Matrigel-based system. Achieved sustained culture for over 350 days with all differentiated epithelial lineages and mesenchymal compartment preservation.
Cell source
Primary (Mouse and Human Intestinal Tissue)
Application
Long-Term Intestinal Culture
No step-by-step SOP yet
OrganMatch™ lists this published method, but there is no step-by-step SOP for it yet.
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