INTESTINAL Publication-derived

Renewal and Cellular Diversity

Source Fujii et al. · Keio University School of Medicine, Tokyo, Japan

👤 Fujii M, Matano M, Toshimitsu K, Takano A, Mikami Y, Nishikori S, Sugimoto S, Sato T ⏱ 28800 min 🧫 Primary (Human Surgical/Biopsy Tissue)

Abstract

Resolved a longstanding challenge in human intestinal organoid culture by developing conditions that maintain full cellular diversity including secretory lineages. Replacing EGF with IGF-1/FGF-2 and removing p38 inhibitor enabled persistent multilineage differentiation over six months.

Cell source

Primary (Human Surgical/Biopsy Tissue)

Application

Improved Organoid Culture

Full SOP

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Attribution

This SOP was authored by Organthis based on the published method in Fujii et al.. The originating laboratory holds no rights in this SOP and has not endorsed it unless marked Verified.

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