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INTESTINAL Publication-derived
Intestinal Organoids (2018)
No SOP yet
Source Fujii et al. · Keio University School of Medicine, Tokyo, Japan
Authors: Fujii M, Matano M, Toshimitsu K, Takano A, Mikami Y, Nishikori S, Sugimoto S, Sato T Duration: 60 days Cell source: Primary (Human Surgical/Biopsy Tissue)
Abstract
Resolved a longstanding challenge in human intestinal organoid culture by developing conditions that maintain full cellular diversity including secretory lineages. Replacing EGF with IGF-1/FGF-2 and removing p38 inhibitor enabled persistent multilineage differentiation over six months.
Cell source
Primary (Human Surgical/Biopsy Tissue)
Application
Improved Organoid Culture
No step-by-step SOP yet
OrganMatch™ lists this published method, but there is no step-by-step SOP for it yet.
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