INTESTINAL Publication-derived

Sequential Cancer Mutations in Cultured Human Intestinal Stem

Source Drost et al. · Hubrecht Institute and University Medical Centre Utrecht, Utrecht, Netherlands

👤 Drost J, van Jaarsveld RH, Ponsioen B, Zimberlin C, van Boxtel R, Buijs A, Sachs N, Overmeer RM, Offerhaus GJ, Begthel H, Korber J, van de Wetering M, Schwank G, Logtenberg M, Cupen E, Snippert HJ, Medema JP, Kops GJPL, Clevers H ⏱ 43200 min 🧫 Primary (Human Normal Intestinal Stem Cells)

Abstract

Demonstrated sequential introduction of the four major CRC driver mutations (APC, P53, KRAS, SMAD4) in normal human intestinal organoids using CRISPR/Cas9. Quadruple mutant organoids acquired niche-independent growth and tumorigenicity, recapitulating the classic adenoma-to-carcinoma progression sequence.

Cell source

Primary (Human Normal Intestinal Stem Cells)

Application

Cancer Progression Modeling

Full SOP

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Attribution

This SOP was authored by Organthis based on the published method in Drost et al.. The originating laboratory holds no rights in this SOP and has not endorsed it unless marked Verified.

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