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INTESTINAL Publication-derived
Intestinal Models for Disease Modelling
No SOP yet
Source Drost et al. · Hubrecht Institute and University Medical Centre Utrecht, Utrecht, Netherlands
Authors: Drost J, van Jaarsveld RH, Ponsioen B, Zimberlin C, van Boxtel R, Buijs A, Sachs N, Overmeer RM, Offerhaus GJ, Begthel H, Korber J, van de Wetering M, Schwank G, Logtenberg M, Cupen E, Snippert HJ, Medema JP, Kops GJPL, Clevers H Duration: 90 days Cell source: Primary (Human Normal Intestinal Stem Cells)
Abstract
Demonstrated sequential introduction of the four major CRC driver mutations (APC, P53, KRAS, SMAD4) in normal human intestinal organoids using CRISPR/Cas9. Quadruple mutant organoids acquired niche-independent growth and tumorigenicity, recapitulating the classic adenoma-to-carcinoma progression sequence.
Cell source
Primary (Human Normal Intestinal Stem Cells)
Application
Cancer Progression Modeling
No step-by-step SOP yet
OrganMatch™ lists this published method, but there is no step-by-step SOP for it yet.
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