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INTESTINAL Publication-derived
Colonic Organoids for Regeneration and Transplantation
No SOP yet
Source Múnera et al. · Cincinnati Children's Hospital Medical Center, Cincinnati, USA · 10.1016/j.stem.2017.05.020
Authors: Múnera JO, Sundaram N, Rankin SA, Hill D, Watson C, Mahe M, Vallance JE, Shroyer NF, Sinagoga KL, Zarzoso-Lacoste A, Hudson JR, Howell JC, Chatber P, Falk P, Zorn AM, Wells JM Duration: 70 days Cell source: Human iPSC / Human ESC
Abstract
Established the first protocol for generating human colonic organoids (HCOs) from iPSCs by adding transient BMP4 signaling during hindgut differentiation. HCOs express colonic markers (SATB2, HOXD13) and lack Paneth cells, enabling region-specific modeling of colonic diseases like ulcerative colitis.
Cell source
Human iPSC / Human ESC
Application
Colonic Disease Modeling
No step-by-step SOP yet
OrganMatch™ lists this published method, but there is no step-by-step SOP for it yet.
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