INTESTINAL Publication-derived

Differentiation of Human PSCs into Colonic Organoids via BMP

Source Múnera et al. · Cincinnati Children's Hospital Medical Center, Cincinnati, USA · 10.1016/j.stem.2017.05.020

👤 Múnera JO, Sundaram N, Rankin SA, Hill D, Watson C, Mahe M, Vallance JE, Shroyer NF, Sinagoga KL, Zarzoso-Lacoste A, Hudson JR, Howell JC, Chatber P, Falk P, Zorn AM, Wells JM ⏱ 33600 min 🧫 Human iPSC / Human ESC

Abstract

Established the first protocol for generating human colonic organoids (HCOs) from iPSCs by adding transient BMP4 signaling during hindgut differentiation. HCOs express colonic markers (SATB2, HOXD13) and lack Paneth cells, enabling region-specific modeling of colonic diseases like ulcerative colitis.

Cell source

Human iPSC / Human ESC

Application

Colonic Disease Modeling

Full SOP

🔬

Create a free account to access this protocol

Join OrganMatch to unlock step-by-step procedures, reagent concentrations, QC checklists, and downloadable batch record templates.

Create free account

Already registered? Log in

Attribution

This SOP was authored by Organthis based on the published method in Múnera et al.. The originating laboratory holds no rights in this SOP and has not endorsed it unless marked Verified.

This wording is awaiting legal review.

Something wrong with this entry? Report an issue with this protocol

Need a commercial licence?

Use this protocol in your therapeutic or diagnostic pipeline.

Request Commercial Licence