INTESTINAL Publication-derived

Derived Intestinal Epithelial Organoids

Source Takahashi et al. · Tokyo Institute of Technology / Shionogi Pharmaceutical, Yokohama/Osaka, Japan · 10.1016/j.stemcr.2017.11.008

👤 Takahashi Y, Sato S, Kurashima Y, Yamamoto T, Kurokawa S, Yuki Y, Takemura N, Uematsu S, Lai CY, Otsu M, Matsuno H, Osawa H, Sato T, Kiyono H ⏱ 24000 min 🧫 Human iPSC

Abstract

Optimized the iPSC-derived intestinal organoid protocol for pharmaceutical drug absorption and metabolism studies. The refined system produces epithelial cells with functional drug transporters (P-gp) and metabolic enzymes (CYP3A4), providing a human-relevant platform for ADME testing.

Cell source

Human iPSC

Application

Drug Absorption Studies

Full SOP

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Attribution

This SOP was authored by Organthis based on the published method in Takahashi et al.. The originating laboratory holds no rights in this SOP and has not endorsed it unless marked Verified.

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