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INTESTINAL Publication-derived
Colonic Models for Developmental Studies
No SOP yet
Source Shimokawa et al. · Keio University School of Medicine, Tokyo, Japan
Authors: Shimokawa M, Ohta Y, Nishikori S, Matano M, Takano A, Fujii M, Date S, Sugimoto S, Kanai T, Sato T Duration: 120 days Cell source: Patient-Derived (CRC Surgical Specimens)
Abstract
Visualized LGR5+ cancer stem cells in human colorectal cancer organoids using CRISPR knock-in reporters. Lineage tracing showed LGR5+ cells drive tumor growth, but their ablation revealed remarkable plasticity where LGR5-negative cells regenerate the stem cell pool, explaining resistance to targeted therapy.
Cell source
Patient-Derived (CRC Surgical Specimens)
Application
Cancer Stem Cell Biology
No step-by-step SOP yet
OrganMatch™ lists this published method, but there is no step-by-step SOP for it yet.
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