INTESTINAL Publication-derived

Visualization and Targeting of LGR5+ Human Colon Cancer Stem

Source Shimokawa et al. · Keio University School of Medicine, Tokyo, Japan

👤 Shimokawa M, Ohta Y, Nishikori S, Matano M, Takano A, Fujii M, Date S, Sugimoto S, Kanai T, Sato T ⏱ 57600 min 🧫 Patient-Derived (CRC Surgical Specimens)

Abstract

Visualized LGR5+ cancer stem cells in human colorectal cancer organoids using CRISPR knock-in reporters. Lineage tracing showed LGR5+ cells drive tumor growth, but their ablation revealed remarkable plasticity where LGR5-negative cells regenerate the stem cell pool, explaining resistance to targeted therapy.

Cell source

Patient-Derived (CRC Surgical Specimens)

Application

Cancer Stem Cell Biology

Full SOP

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Attribution

This SOP was authored by Organthis based on the published method in Shimokawa et al.. The originating laboratory holds no rights in this SOP and has not endorsed it unless marked Verified.

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