INTESTINAL Publication-derived

TNF-α-Driven Epithelial Damage in Intestinal Organoids as IBD

Source Howell et al. · University of Cambridge, UK · 10.1136/gutjnl-2016-312623

👤 Howell KJ, Kraiczy J, Nayak KM, Gasparetto M, Ross A, Lee C, Mber TM, Koo BK, Kumar N, Zilbauer M ⏱ 10080 min 🧫 Primary (Human Intestinal Crypts or iPSC-Derived)

Abstract

Developed a TNF-α-driven intestinal organoid damage model recapitulating key epithelial pathology of IBD including barrier disruption and apoptosis. Anti-TNF biologics rescued damage in a dose-dependent manner, establishing a functional drug screening platform for inflammatory bowel disease therapeutics.

Cell source

Primary (Human Intestinal Crypts or iPSC-Derived)

Application

IBD Drug Screening

Full SOP

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Attribution

This SOP was authored by Organthis based on the published method in Howell et al.. The originating laboratory holds no rights in this SOP and has not endorsed it unless marked Verified.

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