INTESTINAL Publication-derived

Wnt-Responsive Intestinal Organoids from UC Patients Show

Source Nanki et al. · Keio University School of Medicine, Tokyo, Japan

👤 Nanki K, Fujii M, Shimokawa M, Matano M, Nishikori S, Date S, Takano A, Toshimitsu K, Ohta Y, Takahashi S, Sugimoto S, Ishimaru K, Kawasaki K, Togasaki K, Kanai T, Sato T ⏱ 16800 min 🧫 Patient-Derived (UC and Control Colonic Biopsies, Large Cohort)

Abstract

Conducted large-scale organoid phenotyping from UC patients revealing intrinsic stem cell dysfunction including reduced clonogenicity and altered Wnt signaling dependency. Single-cell RNA-seq identified UC-specific stem cell subpopulations with abnormal lineage allocation, establishing stem cell biology as a key axis in UC pathogenesis.

Cell source

Patient-Derived (UC and Control Colonic Biopsies, Large Cohort)

Application

UC Stem Cell Biology

Full SOP

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Attribution

This SOP was authored by Organthis based on the published method in Nanki et al.. The originating laboratory holds no rights in this SOP and has not endorsed it unless marked Verified.

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