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INTESTINAL Publication-derived

Intestine-Chip Enables Rapid Drug Pharmacokinetic and

Source Kasendra et al. · Emulate Inc. and Harvard University, Boston, USA · 10.1038/s41598-018-21201-7

👤 Kasendra M, Tovaglieri A, Sontheimer-Phelps A, Jalili-Firoozinezhad S, Bein A, Chalkiadaki A, Scholl W, Zhang C, Rickner H, Richmond CA, Li H, Breault DT, Ingber DE ⏱ 10080 min 🧫 Primary (Human Intestinal Biopsy-Derived Organoid Cells)

Abstract

Developed a primary human intestine-chip by seeding organoid-derived epithelial cells opposite vascular endothelium in the Emulate platform. The chip's transcriptome more closely resembled in vivo duodenum than organoids or Caco-2, with enhanced CYP3A4 drug metabolism activity supporting physiologically relevant PK/PD studies.

Cell source
Primary (Human Intestinal Biopsy-Derived Organoid Cells)
Application
Drug Absorption & Metabolism

Full SOP

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Attribution

This SOP was authored by Organthis based on the published method in Kasendra et al.. The originating laboratory holds no rights in this SOP and has not endorsed it unless marked Verified.

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