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INTESTINAL Publication-derived
Intestinal Organoids (2017)
No SOP yet
Source Janda et al. · Stanford University School of Medicine, Stanford, USA · 10.1038/nature22306
Authors: Janda CY, Dang LT, You C, Chang J, de Lau W, Zhong ZA, Yan KS, Marecber O, Sumer D, Li X, Waber N, Kim Y, Ber R, Ber Y, Garcia KC, Clevers H, Kuo CJ Duration: 21 days Cell source: Primary (Mouse and Human Intestinal Crypts)
Abstract
Engineered a water-soluble next-generation surrogate Wnt agonist that bypasses the need for lipid-modified Wnt3A in intestinal organoid culture. NGS-Wnt provides equivalent stem cell support with superior stability and batch-to-batch consistency, enabling defined and GMP-compatible organoid culture conditions.
Cell source
Primary (Mouse and Human Intestinal Crypts)
Application
Defined Organoid Culture
No step-by-step SOP yet
OrganMatch™ lists this published method, but there is no step-by-step SOP for it yet.
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