INTESTINAL Publication-derived

Regional Identity of Intestinal Organoids Affects Drug Metabolism

Source Middendorp et al. · University Medical Centre Utrecht and Hubrecht Institute, Netherlands

👤 Middendorp S, Schneeberger K, Wiber S, Nos KM, van de Wetering M, Clevers H, Nieuwenhuis EES ⏱ 13440 min 🧫 Primary (Human Duodenal, Jejunal, Ileal, and Colonic Biopsies)

Abstract

Established organoids from four intestinal regions demonstrating stable retention of regional identity through HOX gene expression and site-specific drug metabolism patterns. Duodenal organoids expressed highest CYP3A4 levels, supporting regional drug metabolism profiling for oral drug development.

Cell source

Primary (Human Duodenal, Jejunal, Ileal, and Colonic Biopsies)

Application

Regional Drug Absorption

Full SOP

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Attribution

This SOP was authored by Organthis based on the published method in Middendorp et al.. The originating laboratory holds no rights in this SOP and has not endorsed it unless marked Verified.

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