INTESTINAL Publication-derived

Region-Specific Human Intestinal Organoids Retain Positional

Source Kraiczy et al. · University of Cambridge and Wellcome Sanger Institute, UK · 10.1016/j.celrep.2019.05.035

👤 Kraiczy J, Nayber KM, Howell KJ, Ross A, Forbester J, Salber GR, Tambber Y, Heber N, Zilbar-Rosenberg I, Rosenberg E, Varga-Weisz P, Jawaid K, Kelly D, Chubb JR, Mahbubani K, Saeb-Parsy K, Jones RL, Koslowski MJ, Rorsman F, Haniffa M, Teichmann SA, Dougan G, Lee JC ⏱ 13440 min 🧫 Primary (Human Duodenal, Jejunal, Ileal, and Colonic Biopsies — Pediatric)

Abstract

Comprehensive multi-omic characterization demonstrating that human intestinal organoids from different regions maintain stable positional identity through DNA methylation-encoded HOX gene programs preserved during long-term culture, validating regional organoid biobanking for site-specific disease modeling.

Cell source

Primary (Human Duodenal, Jejunal, Ileal, and Colonic Biopsies — Pediatric)

Application

Regional Intestinal Biology

Full SOP

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Attribution

This SOP was authored by Organthis based on the published method in Kraiczy et al.. The originating laboratory holds no rights in this SOP and has not endorsed it unless marked Verified.

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