INTESTINAL Publication-derived

Alginate-Based Matrigel-Free Intestinal Organoid Culture

Source Capeling et al. · University of Michigan, Ann Arbor, USA · 10.1016/j.stemcr.2018.12.011

👤 Capeling MM, Czerwinski M, Huang S, Tsai YH, Wu A, Nagy MS, Juliar B, Sundaram N, Song Y, Han WM, Takayama S, Alber E, Garcia AJ, Helmrath MA, Spence JR ⏱ 10080 min 🧫 iPSC-Derived (Human iPSC-Derived HIOs in Alginate)

Abstract

Established alginate hydrogel as a cost-effective Matrigel alternative for iPSC-derived intestinal organoid culture with comparable organoid quality. The alginate bead format enables bioreactor-compatible scalable production using an FDA-approved biomaterial.

Cell source

iPSC-Derived (Human iPSC-Derived HIOs in Alginate)

Application

Scalable Organoid Production

Full SOP

🔬

Create a free account to access this protocol

Join OrganMatch to unlock step-by-step procedures, reagent concentrations, QC checklists, and downloadable batch record templates.

Create free account

Already registered? Log in

Attribution

This SOP was authored by Organthis based on the published method in Capeling et al.. The originating laboratory holds no rights in this SOP and has not endorsed it unless marked Verified.

This wording is awaiting legal review.

Something wrong with this entry? Report an issue with this protocol

Need a commercial licence?

Use this protocol in your therapeutic or diagnostic pipeline.

Request Commercial Licence