INTESTINAL Publication-derived

Suspension Culture of Intestinal Organoids Without ECM

Source Li et al. · University of Cambridge, UK · 10.1038/nm.3585

👤 Li X, Nadauld L, Ootani A, Corney DC, Pai RK, Gevaert O, Cantrell MA, Rack PG, Neal JT, Chan CWM, Yeung T, Gong X, Yuan J, Wilhelmy J, Robber S, Attardi LD, Plevritis SK, Bhatt AS, Kuo CJ ⏱ 10080 min 🧫 Primary (Mouse and Human Intestinal Crypts)

Abstract

Demonstrated intestinal organoid growth in matrix-free suspension culture using air-liquid interface conditions, enabling scalable production in spinner flasks without ECM dependency. Long-term expansion with multilineage differentiation supports industrial manufacturing.

Cell source

Primary (Mouse and Human Intestinal Crypts)

Application

Scalable Production

Full SOP

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Attribution

This SOP was authored by Organthis based on the published method in Li et al.. The originating laboratory holds no rights in this SOP and has not endorsed it unless marked Verified.

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