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INTESTINAL Publication-derived
Cystic Fibrosis Intestinal Organoids
No SOP yet
Source Dekkers et al. · University Medical Centre Utrecht, Netherlands
Authors: Dekkers JF, Wiegerinck CL, de Jonge HR, Bronsveld I, Janssens HM, de Winter-de Groot KM, Brandsma AM, de Jong NWM, Bijvelds MJC, Scholte BJ, Nieuwenhuis EES, van den Brink S, Clevers H, van der Ent CK, Middendorp S, Beekman JM Duration: 14 days Cell source: Primary (Human Rectal Biopsies from CF and Healthy Subjects)
Abstract
Established the original forskolin-induced swelling assay as a direct functional readout of CFTR chloride channel activity in patient-derived rectal organoids. Mutation-specific swelling profiles enable genotype-phenotype correlation and personalized CFTR modulator therapy selection.
Cell source
Primary (Human Rectal Biopsies from CF and Healthy Subjects)
Application
CFTR Functional Assessment
No step-by-step SOP yet
OrganMatch™ lists this published method, but there is no step-by-step SOP for it yet.
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