INTESTINAL Publication-derived

Original CFTR Organoid Functional Test

Source Dekkers et al. · University Medical Centre Utrecht, Netherlands

👤 Dekkers JF, Wiegerinck CL, de Jonge HR, Bronsveld I, Janssens HM, de Winter-de Groot KM, Brandsma AM, de Jong NWM, Bijvelds MJC, Scholte BJ, Nieuwenhuis EES, van den Brink S, Clevers H, van der Ent CK, Middendorp S, Beekman JM ⏱ 6720 min 🧫 Primary (Human Rectal Biopsies from CF and Healthy Subjects)

Abstract

Established the original forskolin-induced swelling assay as a direct functional readout of CFTR chloride channel activity in patient-derived rectal organoids. Mutation-specific swelling profiles enable genotype-phenotype correlation and personalized CFTR modulator therapy selection.

Cell source

Primary (Human Rectal Biopsies from CF and Healthy Subjects)

Application

CFTR Functional Assessment

Full SOP

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Attribution

This SOP was authored by Organthis based on the published method in Dekkers et al.. The originating laboratory holds no rights in this SOP and has not endorsed it unless marked Verified.

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