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INTESTINAL Publication-derived
Cystic Fibrosis Intestinal Organoids for Developmental Studies
No SOP yet
Source Schwank et al. · Hubrecht Institute, Utrecht, Netherlands
Authors: Schwank G, Koo BK, Sasselli V, Dekkers JF, Heo I, Demircan T, Sasaki N, Boymans S, Cuppen E, van der Ent CK, Nieuwenhuis EES, Beekman JM, Clevers H Duration: 42 days Cell source: Primary (Human CF Patient Intestinal Organoids, F508del/F508del)
Abstract
Achieved the first CRISPR-Cas9 correction of the CFTR F508del mutation in patient-derived intestinal organoids, restoring functional CFTR chloride channel activity as demonstrated by forskolin-induced swelling. This landmark proof-of-concept established organoid gene therapy feasibility.
Cell source
Primary (Human CF Patient Intestinal Organoids, F508del/F508del)
Application
Gene Therapy Development
No step-by-step SOP yet
OrganMatch™ lists this published method, but there is no step-by-step SOP for it yet.
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