INTESTINAL Publication-derived

Tubular Intestinal Organoid in Microfluidic Device

Source Nikolaev et al. · EPFL, Lausanne, Switzerland

👤 Nikolaev M, Mitrofanova O, Broguiere N, Gerber S, Bber D, Kober L, Prados J, Osteil P, Bartber R, Harper JL, Lutolf MP ⏱ 10080 min 🧫 Primary (Mouse and Human Intestinal Crypts)

Abstract

Created centimeter-scale tubular intestinal organoids with in vivo-like crypt-villus architecture through guided self-organization in microengineered collagen scaffolds with morphogen gradients, achieving months-long stable culture with the most physiological intestinal organoid architecture to date.

Cell source

Primary (Mouse and Human Intestinal Crypts)

Application

Physiological Gut Models

Full SOP

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Attribution

This SOP was authored by Organthis based on the published method in Nikolaev et al.. The originating laboratory holds no rights in this SOP and has not endorsed it unless marked Verified.

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