LIVER Publication-derived

Cholangiocyte Organoids Repair Human Bile Ducts

Source Sampaziotis et al. · University of Cambridge / Cambridge University Hospitals NHS · 10.1126/science.aaz6964

👤 Sampaziotis F, Muraro D, Tysoe OC, Sawiak S, Beach TE, Godfrey EM, Upponi SS, Brevini T, Wesley BT, Vallier L ⏱ 20160 min 🧫 Human Primary Cholangiocyte Organoids → Normothermic Perfused Donor Liver

Abstract

Sampaziotis et al. (Science 2021) lieferten den ersten Beweis, dass Cholangiocyten-Organoids menschliche Gallengänge reparieren können. ECOs wurden in die Gallengänge von ex vivo normotherm perfundierten humanen Spenderlebern transplantiert (bis zu 100h Perfusion). Engraftment und funktionale Reparatur des Gallengangsepithels wurden durch Histologie und scRNA-seq bestätigt. Dieser Meilenstein bildet die Grundlage für laufende klinische Phase-I-Studien zur Organoid-basierten Therapie von Cholangiopathien.

Cell source

Human Primary Cholangiocyte Organoids → Normothermic Perfused Donor Liver

Application

Biliary Cell Therapy

Full SOP

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Attribution

This SOP was authored by Organthis based on the published method in Sampaziotis et al.. The originating laboratory holds no rights in this SOP and has not endorsed it unless marked Verified.

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