LIVER Publication-derived

Kupffer-like Coculture (Lee/Son et al.)

Source Lee et al. · KRIBB, Daejeon / Institute Pasteur Korea

👤 Lee J, Gil D, Park H, Lee Y, Mun SJ, Shin Y, Jo E, Windisch MP, Kim JH, Son MJ ⏱ 19200 min 🧫 Human PSC → Hepatocytes + Kupffer-like Macrophages (Coculture)

Abstract

Lee und Son et al. (KRIBB Korea) präsentierten ein multicellulares Organoidmodell aus PSC-abgeleiteten Hepatozyten und Kupffer-ähnlichen Makrophagen für die Co-Modellierung von HCV und NAFLD. HCV-Infektion induzierte Lipogenese und verstärkte Fettakkumulation in Gegenwart von NAFLD-Stimulus. Kupffer-ähnliche Makrophagen verstärkten die Entzündungsantwort synergistisch. Erstes Modell für die klinisch häufige Dual-Pathologie HCV + NAFLD.

Cell source

Human PSC → Hepatocytes + Kupffer-like Macrophages (Coculture)

Application

HCV + NAFLD Co-Pathology Modeling

Full SOP

🔬

Create a free account to access this protocol

Join OrganMatch to unlock step-by-step procedures, reagent concentrations, QC checklists, and downloadable batch record templates.

Create free account

Already registered? Log in

Attribution

This SOP was authored by Organthis based on the published method in Lee et al.. The originating laboratory holds no rights in this SOP and has not endorsed it unless marked Verified.

This wording is awaiting legal review.

Something wrong with this entry? Report an issue with this protocol

Need a commercial licence?

Use this protocol in your therapeutic or diagnostic pipeline.

Request Commercial Licence